Granulocyte colony stimulating factor (GCSF) protected in ovarian tissues against ischemia-reperfusion injury

Ovarian torsion is an important reproductive gynecological emergency in daily life and physiology. Granulocyte colony stimulating factor (G-CSF) a glycoprotein capable of hematopoietic development successfully used the treatment congenital granulocytopenia. In our study, we aimed to investigate protective effects G-CSF on ovarian ischemia reperfusion (IR) immunohistochemically. Four groups were used. control group, abdomen was opened closed with surgical protocol. The rats group given 100 µg/kg subcutaneously before IR surgically closed. 3-h then induced ischemia-reperfusion (I/R) group. IR+G-CSF procedure performed. At end experiment, tissues fixed 10% formalin processed for routine paraffin tissue Normal histology observed groups. vascular dilatations, hemorrhage increased inflammation observed. I/R+G-CSF pathology seen decreased. IL-6 expression mainly negative Positive immune reaction granulosa cells stromal area. significantly decreased follicular structures area compared I/R conclusion, reduced dilatation model promoted folliculogenesis.
 Keywords: Ovary, Ischemia/reperfusion, G-CSF, Azan, IL-6, Immunohistochemistry